Single Use Bioreactor diffuser sparger for cell culture

In the initial stage of upstream processing in bioprocessing, fermentation is commonly used. Fermentation is defined as the chemical changes caused by microorganisms in the production of pharmaceuticals and other biotechnology products. This process typically takes place in bioreactors or fermenters, depending on the culture medium used. These bioreactor vessels allow for strict monitoring and control of microorganisms (or mammalian cells) growth and product synthesis.

Fermentation is the cornerstone of the final product production in the bioprocessing industry.

Bioprocessing and companies focused on cell- and gene-based therapy products understand that optimal microbial synthesis conditions must be provided during fermentation applications. In addition, the fermentation process must help ensure economically feasible yields, enabling cost-effective large-scale production.Modern bioprocessing facilities have a volume range of 1,000 to 25,000 liters. Scaling up biological materials from a few million cells in a few milliliters of culture to these production yields is a challenge that requires sterile culture medium transfer at every point of seed cultivation.

Disposable technologies, also known as Single-Use Technologies (SUT), can greatly enhance fermentation efficiency.

The advanced nature of SUT allows bioprocess engineers to choose disposable systems and delivery pipelines to replace storage vessels and fixed pipeline networks. With the increasing popularity of SUT, bioprocessing manufacturers can design flexible seed cultivation systems comprising of single-use bioreactors (SUBs) of various volumes or use a combination of SUBs and traditional stainless steel reactors to achieve super-sized production. The use of CPC aseptic couplings or sanitary equipment couplings, equipped with in situ steam-in-place (SIP) connectors, allows for easy and safe transfer of culture medium during the connection of various components.

Disposable sterile bioreactor aeration stones are designed for bioreactor fermentation process

HENGKO Producer deploys a wide range of disposable stainless steel sintered filter components to create the fermentation and production processes our customers expect. Hengge offers the widest range of single-use connection solutions, reliably combining these technologies while maintaining the sterility and integrity of the overall system.

Versatility and Flexibility
HENGER's sterile aeration stones are flexible in that product construction and appearance and connector components can be custom designed and manufactured to meet customer needs.

Innovative Design
Our team of bioprocessing design and application engineers is focused on developing innovative, high-quality products that make transferring sterile media safe and easy. As the fermentation effect of a reactor varies depending on the choice of filter, hengko's sterile aeration stones are available in a wide range of sizes, configurations, end options and materials and are made from high quality raw materials. Innovative products ensure ease of use and connection assurance. Reliability you can trust

Reliability you can trust
Aeration Stones are built for all bioprocessing applications in bioreactors. By helping to maintain the sterile transfer of media, aeration stones help to achieve optimal yields at a cost-effective price. All aeration stone solutions are rigorously tested (including material testing, product testing and product stability testing) to meet product specifications. In fact, hengko provides reliable, reproducible performance with validation of supporting properties and extractable reports.

Typical application
– Production of vaccines, recombinant proteins and monoclonal antibodies
production process development
– Production of biofuels and secondary metabolites
process development
– for batch, batch, continuous or perfusion operation
process technology development
– Scaling up and downscaling of trial size
– Small-scale production, such as diagnostic antibodies
– High cell density fermentation
– Suspension culture and adherence using microcarriers
cell culture
– Culture of filamentous microbes